Deleted
Sample Discontinued
Experience: None
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Plasmid

Part:BBa_K1682010:Design

Designed by: Zilu Peter Tang   Group: iGEM15_HKUST-Rice   (2015-08-26)


conjugation(RP4) plasmid with MHT (Methyl halide transferase) and GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1780
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2157
    Illegal NgoMIV site found at 2283
    Illegal NgoMIV site found at 2293
    Illegal AgeI site found at 1592
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1814
    Illegal SapI site found at 2185


Design Notes

N/A


Source

MHT sequence comes from organism Batis maritima. GFP comes from the biobrick BBa_E0040. RP4 comes from gBlock pRZ101

References